In:
Science Advances, American Association for the Advancement of Science (AAAS), Vol. 7, No. 5 ( 2021-01-29)
Abstract:
Natural genetic variation affects circadian rhythms across the evolutionary tree, but the underlying molecular mechanisms are poorly understood. We investigated population-level, molecular circadian clock variation by generating 〉 700 tissue-specific transcriptomes of Drosophila melanogaster ( w 1118 ) and 141 Drosophila Genetic Reference Panel (DGRP) lines. This comprehensive circadian gene expression atlas contains 〉 1700 cycling genes including previously unknown central circadian clock components and tissue-specific regulators. Furthermore, 〉 30% of DGRP lines exhibited aberrant circadian gene expression, revealing abundant genetic variation–mediated, intertissue circadian expression desynchrony. Genetic analysis of one line with the strongest deviating circadian expression uncovered a novel cry mutation that, as shown by protein structural modeling and brain immunohistochemistry, disrupts the light-driven flavin adenine dinucleotide cofactor photoreduction, providing in vivo support for the importance of this conserved photoentrainment mechanism. Together, our study revealed pervasive tissue-specific circadian expression variation with genetic variants acting upon tissue-specific regulatory networks to generate local gene expression oscillations.
Type of Medium:
Online Resource
ISSN:
2375-2548
DOI:
10.1126/sciadv.abc3781
Language:
English
Publisher:
American Association for the Advancement of Science (AAAS)
Publication Date:
2021
detail.hit.zdb_id:
2810933-8