In:
Science Immunology, American Association for the Advancement of Science (AAAS), Vol. 5, No. 45 ( 2020-03-13)
Abstract:
B cells in human food allergy have been studied predominantly in the blood. Little is known about IgE + B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE + clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE + cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE + and non-IgE–expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE + B lineage cells in food allergy.
Type of Medium:
Online Resource
ISSN:
2470-9468
DOI:
10.1126/sciimmunol.aay4209
Language:
English
Publisher:
American Association for the Advancement of Science (AAAS)
Publication Date:
2020
