In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 61, No. 6 ( 2017-06)
Abstract:
A PCR-dipstick chromatography technique was designed and evaluated for differential identification of bla NDM , bla KPC , bla IMP , and bla OXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.00067-17
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2017
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3