In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 43, No. 10 ( 1999-10), p. 2493-2496
Abstract:
The role of mutations in the genes for GyrA and ParC in quinolone resistance in Mycoplasma hominis was studied. Selection with sparfloxacin gave mutations at GyrA83 (Ser→Leu; Escherichia coli numbering) or GyrA87 (Glu→Lys), and mutants had increased levels of resistance to sparfloxacin (8- to 16-fold) but not to ofloxacin. Selection with ofloxacin gave changes at ParC80 (Ser→Ile) or ParC84 (Glu→Lys), and mutants were four- to eightfold more resistant to ofloxacin but not to sparfloxacin. Selection of second-step mutants from strains with ParC mutations with either quinolone yielded double mutants with additional mutations at GyrA83 (Ser→Trp or Ser→Leu) or GyrA87 (Glu→Lys). Second-step selection of GyrA mutants gave additional mutations at ParC80 (Ser→Ile) or ParC84 (Glu→Lys). Two-step mutants showed high levels of resistance to ofloxacin (MICs, 64 to 128 μg/ml) and moderate levels of resistance to sparfloxacin (MICs, 2 to 8 μg/ml). The primary target of ofloxacin in first-step mutants of Mycoplasma hominis was ParC, whereas that for sparfloxacin was GyrA.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.43.10.2493
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1999
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3