In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 43, No. 5 ( 1999-05), p. 1211-1214
Abstract:
Biofilm infections are a common complication of prosthetic devices in humans. Previous in vitro research has determined that low-frequency ultrasound combined with aminoglycoside antibiotics is an effective method of killing biofilms. We report the development of an in vivo model to determine if ultrasound enhances antibiotic action. Two 24-h-old Escherichia coli (ATCC 10798) biofilms grown on polyethylene disks were implanted subcutaneously on the backs of New Zealand White female rabbits, one on each side of the spine. Low-frequency (28.48-kHz) and low-power-density (100- and 300-mW/cm 2 ) continuous ultrasound treatment was applied for 24 h with and without systemic administration of gentamicin. The disks were then removed, and the number of viable bacteria on each disk was determined. At the low ultrasonic power used in this study, exposure to ultrasound only (no gentamicin) caused no significant difference in bacterial viability. In the presence of antibiotic, there was a significant reduction due to 300-mW/cm 2 ultrasound ( P = 0.0485) but no significant reduction due to 100-mW/cm 2 ultrasound. Tissue damage to the skin was noted at the 300-mW/cm 2 treatment level. Further development of this technique has promise in treatment of clinical implant infections.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.43.5.1211
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1999
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
