In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 48, No. 1 ( 2004-01), p. 209-215
Abstract:
Sequencing of the upstream region of the β-lactamase gene from Xanthomonas campestris pv. campestris 11 ( bla XCC-1 ) revealed the cognate ampR1 gene (289 amino acids, 31 kDa). It runs divergently from bla XCC-1 with a 100-bp intergenic region (IG) containing partially overlapped promoters with structural features typical of the bla - ampR IG. The deduced AmpR1 protein shows significant identity in amino acid sequence and conserved motifs with AmpR proteins of other species, e.g., of Pseudomonas aeruginosa (58.2% amino acid identity). Results of insertional mutation, complementation tests, and β-lactamase assays suggested that expression of bla XCC-1 was constitutive and dependent on AmpR1. Four bla genes and two ampR genes are present in the fully sequenced X. campestris pv. campestris ATCC 33913 genome, with XCC3039 and XCC3040 considered the analogues of bla XCC-1 and ampR1 , respectively. An ampR1 homologue was detected by Southern hybridization in the ampicillin-resistant Xanthomonas strains, which appear to express β-lactamase constitutively. Although the significance remains to be studied, constitutive expression of β-lactamase by a widespread bacterial genus raises environmental concerns regarding the dissemination of resistance genes.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.48.1.209-215.2004
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2004
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
