In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 64, No. 6 ( 1998-06), p. 2061-2064
Abstract:
Four marine bacteria, Alteromonas sp. strains A27, A28, A29, and A30, that lyse the diatom Skeletonema costatum NIES-324 were isolated from coastal seawater samples. They were also able to lyse the diatoms Thalassiosira sp. and Eucampia zodiacs and the raphidophycean flagellate Chattonella antiqua . Cryptic indigenous plasmids, designated pAS28 and pAS29, were detected in Alteromonas sp. strains A28 and A29, respectively. These plasmids appeared to be similar based on size and restriction site analysis. A shuttle vector that replicates in Escherichia coli and Alteromonas sp. strain A28 was constructed by fusing pAS28 and E. coli vector pCRIIc. The 16-kbp chimeric plasmid, designated pASS1, had the ability to transform strain A28 at a frequency of 10 6 transformants per μg of DNA. Deletion analysis of pASS1 showed that the 4.7-kb Eco RI- Hin dIII region of pAS28 was essential for plasmid maintenance in strain A28. This Eco RI- Hin dIII fragment contained an open reading frame which appeared to encode a 708-amino-acid protein.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.64.6.2061-2064.1998
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1998
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
