In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 68, No. 11 ( 2002-11), p. 5241-5248
Kurzfassung:
Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the production of glucosylceramide with this bacterium. GM1a ganglioside was found to be sequentially degraded by Paenibacillus sp. strain TS12, which was isolated from soil, as follows: GM1a → asialo GM1 → asialo GM2 → lactosylceramide → glucosylceramide. TS12 was found to produce a series of ganglioside-degrading enzymes, such as sialidases, β-galactosidases, and β-hexosaminidases. TS12 also produced β-glucosidases, but glucosylceramide was somewhat resistant to the bacterial enzyme under the conditions used. Taking advantage of the specificity, we developed a new method for the production of glucosylceramide using TS12 as a biocatalyst. The method involves the conversion of crude bovine brain gangliosides to glucosylceramide by coculture with TS12 and purification of the product by chromatography with Wakogel C-300 HG.
Materialart:
Online-Ressource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.68.11.5241-5248.2002
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
2002
ZDB Id:
223011-2
ZDB Id:
1478346-0
SSG:
12
