In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 69, No. 9 ( 2003-09), p. 5574-5584
Abstract:
The complete nucleotide sequence of the 13-kb plasmid pRV500, isolated from Lactobacillus sakei RV332, was determined. Sequence analysis enabled the identification of genes coding for a putative type I restriction-modification system, two genes coding for putative recombinases of the integrase family, and a region likely involved in replication. The structural features of this region, comprising a putative ori segment containing 11- and 22-bp repeats and a repA gene coding for a putative initiator protein, indicated that pRV500 belongs to the pUCL287 subfamily of theta-type replicons. A 3.7-kb fragment encompassing this region was fused to an Escherichia coli replicon to produce the shuttle vector pRV566 and was observed to be functional in L . sakei for plasmid replication. The L . sakei replicon alone could not support replication in E . coli . Plasmid pRV500 and its derivative pRV566 were determined to be at very low copy numbers in L . sakei . pRV566 was maintained at a reasonable rate over 20 generations in several lactobacilli, such as Lactobacillus curvatus , Lactobacillus casei , and Lactobacillus plantarum , in addition to L . sakei , making it an interesting basis for developing vectors. Sequence relationships with other plasmids are described and discussed.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.69.9.5574-5584.2003
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2003
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
