In:
Eukaryotic Cell, American Society for Microbiology, Vol. 3, No. 5 ( 2004-10), p. 1359-1362
Abstract:
A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans . The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans .
Type of Medium:
Online Resource
ISSN:
1535-9778
,
1535-9786
DOI:
10.1128/EC.3.5.1359-1362.2004
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2004
detail.hit.zdb_id:
2071564-X
SSG:
12