In:
Infection and Immunity, American Society for Microbiology, Vol. 67, No. 1 ( 1999-01), p. 342-349
Kurzfassung:
Merozoite surface protein 1 is a candidate for blood-stage vaccines against malaria parasites. We report here an immunization study of Saimiri monkeys with a yeast-expressed recombinant protein containing the C terminus of Plasmodium vivax merozoite surface protein 1 and two T-helper epitopes of tetanus toxin (yP 2 P 30 Pv200 19 ), formulated in aluminum hydroxide (alum) and block copolymer P1005. Monkeys immunized three times with yP 2 P 30 Pv200 19 in block copolymer P1005 had significantly higher prechallenge titers of immunoglobulin G (IgG) antibodies against the immunogen and asexual blood-stage parasites than those immunized with yP 2 P 30 Pv200 19 in alum, antigen alone, or phosphate-buffered saline (PBS) ( P 〈 0.05). Their peripheral blood mononuclear cell proliferative responses to immunogen stimulation 4 weeks after the second immunization were also significantly higher than those from the PBS control group ( P 〈 0.05). Upon challenge with 100,000 asexual blood-stage parasites 5 weeks after the last immunization, monkeys immunized with yP 2 P 30 Pv200 19 in block copolymer P1005 had prepatent periods longer than those for the control alone group ( P 〉 0.05). Three of the five animals in this group also had low parasitemia (peak parasitemia, ≤20 parasites/μl of blood). Partially protected monkeys had significantly higher levels of prechallenge antibodies against the immunogen than those unprotected ( P 〈 0.05). There was also a positive correlation between the prepatent period and titers of IgG antibodies against the immunogen and asexual blood-stage parasites and a negative correlation between accumulated parasitemia and titers of IgG antibodies against the immunogen ( P 〈 0.05). These results indicate that when combined with block copolymer and potent T-helper epitopes, the yeast-expressed P 2 P 30 Pv200 19 recombinant protein may offer some protection against malaria.
Materialart:
Online-Ressource
ISSN:
0019-9567
,
1098-5522
DOI:
10.1128/IAI.67.1.342-349.1999
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
1999
ZDB Id:
1483247-1
