In:
Journal of Bacteriology, American Society for Microbiology, Vol. 180, No. 17 ( 1998-09), p. 4734-4738
Abstract:
A gene encoding β-galactosidase, designated mbgA , was isolated from Bacillus megaterium ATCC 14581. Chromosomal β-galactosidase production could be dramatically induced by lactose but not by isopropyl-β- d -thiogalactopyranoside (IPTG) and was subject to catabolite repression by glucose. Disruption of mbgA in the B. megaterium chromosome resulted in loss of lactose-inducible β-galactosidase production. A 27-bp inverted repeat was found to overlap the mbgA promoter sequence. Two partially overlapping catabolite-responsive elements (CREs) were identified within the inverted repeat. Base substitutions within CRE-I and/or CRE-II caused partial relief from catabolite repression. The results suggest that the 27-bp inverted repeat may serve as a target for a catabolite repressor(s).
Type of Medium:
Online Resource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/JB.180.17.4734-4738.1998
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1998
detail.hit.zdb_id:
1481988-0
SSG:
12