In:
Journal of Bacteriology, American Society for Microbiology, Vol. 183, No. 20 ( 2001-10-15), p. 5870-5876
Kurzfassung:
The Escherichia coli osmC gene encodes an envelope protein of unknown function whose expression depends on osmotic pressure and growth phase. The gene is transcribed from two overlapping promoters, osmCp 1 and osmCp 2 . Several factors regulating these promoters have been reported. The leucine-responsive protein Lrp represses osmCp 1 and activates osmCp 2 , the nucleoid-associated protein H-NS represses both promoters, and the stationary-phase sigma factor ς s specifically recognizes osmCp 2 . This work reports the identification of an additional regulatory element, the two-component system rcsB - rcsC , affecting positively the distal promoter osmCp 1 . The response regulator of the system, RcsB, does not affect expression of the proximal promoter osmCp 2 . Deletion analysis located the site necessary for RcsB activation just upstream of osmCp 1 . In vitro transcription experiments and gel mobility shift assays demonstrated that RcsB stimulates RNA polymerase binding at osmCp 1 .
Materialart:
Online-Ressource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/JB.183.20.5870-5876.2001
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
2001
ZDB Id:
1481988-0
SSG:
12
