In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 44, No. 9 ( 2006-09), p. 3443-3447
Kurzfassung:
We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.
Materialart:
Online-Ressource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.01175-06
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
2006
ZDB Id:
1498353-9
SSG:
12
