In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 55, No. 1 ( 2017-01), p. 110-121
Kurzfassung:
New diagnostic platforms often use nasopharyngeal or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multipathogen testing to high-quality sputum specimens to determine if more pathogens can be identified relative to NP/OP swabs. Children ( 〈 18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-quality sputum (defined as ≤10 epithelial cells/low-power field [lpf] and ≥25 white blood cells/lpf or a quality score [q-score] definition of 2+) were tested by TaqMan array card (TAC), a multipathogen real-time PCR detection platform. Among 236 patients with matched specimens, a higher proportion of sputum specimens had ≥1 pathogen detected compared with NP/OP specimens in children (93% versus 68%; P 〈 0.0001) and adults (88% versus 61%; P 〈 0.0001); for each pathogen targeted, crossing threshold ( C T ) values were earlier in sputum. Both bacterial (361 versus 294) and viral detections (245 versus 140) were more common in sputum versus NP/OP specimens, respectively, in both children and adults. When available, high-quality sputum may be useful for testing in hospitalized CAP patients.
Materialart:
Online-Ressource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.01805-16
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
2017
ZDB Id:
1498353-9
SSG:
12
