In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 39, No. 5 ( 2001-05), p. 2017-2021
Abstract:
A group of 1,623 ovine fecal samples recovered from 65 geographically distinct mutton sheep and prime lamb properties across New South Wales, Australia, were screened for the presence of Shiga toxin-producing Escherichia coli (STEC) virulence factors ( stx 1 , stx 2 , eaeA , and ehxA ). A subset was cultured for STEC isolates containing associated virulence factors ( eaeA and/or ehxA ), which were isolated from 17 of 20 (85%) and 19 of 20 (95%) tested prime lamb and mutton sheep properties, respectively. STEC isolates containing stx 1 , stx 2 , and ehxA were most commonly isolated (19 of 40 flocks; 47.5%), and this profile was observed for 10 different serotypes. Among 90 STEC isolates studied, the most common serotypes were O91:H − (22 isolates [24.4%]), O5:H − (16 isolates [17.8%]), O128:H2 (11 isolates [12.2%] ), O123:H − (8 isolates [8.9%]), and O85:H49 (5 isolates [5.6%] ). Two isolates (2.2%) were typed as O157:H − . A total of 78 of 90 STEC isolates (86.7%) expressed Shiga toxin in Vero cell culture and 75 of 84 ehxA -positive isolates (89.3%) expressed enterohemolysin on washed sheep blood agar. eaeA was observed in 11 of 90 (12.2%) ovine STEC isolates, including serotypes O5:H − , O84:H − , O85:H49, O123:H − O136:H40, and O157:H − . Although only 2 of 90 isolates were typed as O157:H − , the predominant serotypes recovered during this study have been recovered from human patients with clinical disease, albeit rarely.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.39.5.2017-2021.2001
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2001
detail.hit.zdb_id:
1498353-9
SSG:
12
