In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 40, No. 5 ( 2002-05), p. 1743-1748
Abstract:
We evaluated two new automated identification systems, the BD Phoenix (Becton Dickinson) and the VITEK 2 (bioMérieux), for identification of isolates of the Burkholderia cepacia complex (BCC). The test sample included 42 isolates of the highly virulent and epidemic genomovar III, 45 isolates of B. multivorans , and 47 isolates of other members of the BCC. Rates of correct identification by the BD Phoenix and VITEK 2 were similar when all BCC isolates were considered (50 and 53%, respectively) but differed markedly for genomovar III (71 and 38%; P 〈 0.01) and for B. multivorans (58 and 89%; P 〈 0.001). For the BD Phoenix as well as the VITEK 2, taking all 134 isolates of the BCC together, rates of correct identification of clinical isolates (56 and 55%, respectively; n = 85) were higher than those of environmental isolates (21 and 39%, respectively; n = 28). Clinical isolates of genomovar III ( n = 27) showed correct identification rates of 81% (BD Phoenix) and 48% (VITEK 2) ( P 〈 0.01). Rates of misidentification for BD Phoenix and VITEK 2 were 9 and 17% for genomovar III, 22 and 7% for B. multivorans , and 36 and 13% for the other BCC members ( P 〈 0.01), respectively. More than half of the isolates misidentified by each instrument were identified as Ralstonia pickettii , Ralstonia paucula (CDC IV C-2 group), Alcaligenes faecalis , Achromobacter spp., or, for the VITEK 2, “various nonfermenters.” This study reemphasizes that confirmatory identification of BCC, preferably by molecular methods, is highly recommended.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.40.5.1743-1748.2002
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2002
detail.hit.zdb_id:
1498353-9
SSG:
12
