In:
Journal of Virology, American Society for Microbiology, Vol. 84, No. 8 ( 2010-04-15), p. 3868-3878
Kurzfassung:
Cytotoxicity and proliferation capacity are key functions of antiviral CD8 T cells. In the present study, we investigated a series of markers to define these functions in virus-specific CD8 T cells. We provide evidence that there is a lack of coexpression of perforin and CD127 in human CD8 T cells. CD127 expression on virus-specific CD8 T cells correlated positively with proliferation capacity and negatively with perforin expression and cytotoxicity. Influenza virus-, cytomegalovirus-, and Epstein-Barr virus/human immunodeficiency virus type 1-specific CD8 T cells were predominantly composed of CD127 + perforin − /CD127 − perforin + , and CD127 − /perforin − CD8 T cells, respectively. CD127 − /perforin − and CD127 − /perforin + cells expressed significantly more PD-1 and CD57, respectively. Consistently, intracellular cytokine (gamma interferon, tumor necrosis factor alpha, and interleukin-2 [IL-2]) responses combined to perforin detection confirmed that virus-specific CD8 T cells were mostly composed of either perforin + /IL-2 − or perforin − /IL-2 + cells. In addition, perforin expression and IL-2 secretion were negatively correlated in virus-specific CD8 T cells ( P 〈 0.01). As previously shown for perforin, changes in antigen exposure modulated also CD127 expression. Based on the above results, proliferating (CD127 + /IL-2-secreting) and cytotoxic (perforin + ) CD8 T cells were contained within phenotypically distinct T-cell populations at different stages of activation or differentiation and showed different levels of exhaustion and senescence. Furthermore, the composition of proliferating and cytotoxic CD8 T cells for a given antiviral CD8 T-cell population appeared to be influenced by antigen exposure. These results advance our understanding of the relationship between cytotoxicity, proliferation capacity, the levels of senescence and exhaustion, and antigen exposure of antiviral memory CD8 T cells.
Materialart:
Online-Ressource
ISSN:
0022-538X
,
1098-5514
DOI:
10.1128/JVI.02565-09
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
2010
ZDB Id:
1495529-5
