In:
Molecular and Cellular Biology, Informa UK Limited, Vol. 10, No. 8 ( 1990-08), p. 4100-4109
Abstract:
AKR leukemias display different amounts of major histocompatibility complex class I antigens on the cell surface. The absence of H-2Kk molecules correlates with the ability of these cell lines to form tumors in vivo as well as to escape lysis by cytotoxic T lymphocytes in vitro. In this report it is shown that the 5' regulatory area of the H-2Kk gene failed to activate transcription in H-2Kk-negative cells. Examination of the proteins interacting with the H-2Kk enhancer in expressing and nonexpressing cells revealed clear differences. In particular, the level of a nuclear protein interacting at position -166 was greatly reduced in the negative cell lines. A transcription factor, known as H2TF1 or KBF1, has been shown previously to interact with this binding site and to be essential for the expression of certain class I genes as well as the expression of beta 2-microglobulin. These results demonstrate that the molecular mechanism of class I gene suppression in malignant tumor cells is at the level of transcription and is most probably modulated by H2TF1/KBFI. In addition, it is shown that the same transcription factor is only present in mouse tissues expressing class I antigens.
Type of Medium:
Online Resource
ISSN:
0270-7306
,
1098-5549
DOI:
10.1128/MCB.10.8.4100
Language:
English
Publisher:
Informa UK Limited
Publication Date:
1990
detail.hit.zdb_id:
1474919-1
SSG:
12
