In:
Molecular and Cellular Biology, Informa UK Limited, Vol. 12, No. 10 ( 1992-10), p. 4314-4326
Abstract:
RPC53 is shown to be an essential gene encoding the C53 subunit specifically associated with yeast RNA polymerase C (III). Temperature-sensitive rpc53 mutants were generated and showed a rapid inhibition of tRNA synthesis after transfer to the restrictive temperature. Unexpectedly, the rpc53 mutants preferentially arrested their cell division in the G1 phase as large, round, unbudded cells. The RPC53 DNA sequence is predicted to code for a hydrophilic M(r)-46,916 protein enriched in charged amino acid residues. The carboxy-terminal 136 amino acids of C53 are significantly similar (25% identical amino acid residues) to the same region of the human BN51 protein. The BN51 cDNA was originally isolated by its ability to complement a temperature-sensitive hamster cell mutant that undergoes a G1 cell division arrest, as is true for the rpc53 mutants.
Type of Medium:
Online Resource
ISSN:
0270-7306
,
1098-5549
DOI:
10.1128/MCB.12.10.4314
Language:
English
Publisher:
Informa UK Limited
Publication Date:
1992
detail.hit.zdb_id:
1474919-1
SSG:
12