In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 47, No. 2 ( 1984-02), p. 403-408
Kurzfassung:
The kinetics of sulfate and acetate uptake was studied in the sulfate-reducing bacterium Desulfobacter postgatei (DSM 2034). Kinetic parameters ( K m and V max ) were estimated from substrate consumption curves by resting cell suspensions with [ 35 S]sulfate and [ 14 C]acetate. Both sulfate and acetate consumption followed Michaelis-Menten saturation kinetics. The half-saturation constant ( K m ) for acetate uptake was 70 μM with cells from either long-term sulfate- or long-term acetate-limited chemostat cultures. The average K m value for sulfate uptake by D. postgatei was about 200 μM. K m values for sulfate uptake did not differ significantly when determined with cells derived either from batch cultures or sulfate- or acetate-limited chemostat cultures. Acetate consumption was observed at acetate concentrations of ≤1 μM, whereas sulfate uptake usually ceased at 5 to 20 μM. The results show that D. postgatei is not freely permeable to sulfate ions and further indicate that sulfate uptake is an energy-requiring process.
Materialart:
Online-Ressource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/aem.47.2.403-408.1984
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
1984
ZDB Id:
223011-2
ZDB Id:
1478346-0
SSG:
12
