In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 59, No. 4 ( 1993-04), p. 1176-1180
Abstract:
A Mo 6+ -reducing bacterium (strain 48), which grew on medium supplemented with 200 mM Mo 6+ , was isolated from stream water obtained from Chengkau, Malaysia. The chemical properties of strain 48 conform to the characteristics of Enterobacter cloacae . Under anaerobic conditions in the glucose-yeast extract medium containing phosphate ion (2.9 mM) and Mo 6+ (10 mM), the bacterium reduced Mo 6+ to form molybdenum blue. Approximately 27% of Mo 6+ added to the medium was reduced after 28 h of cultivation. The reduction of Mo 6+ with glucose as an electron donor was strongly inhibited by iodoacetic acid, sodium fluoride, and sodium cyanide, suggesting an involvement of the glycolytic pathway and electron transport in Mo 6+ reduction. NADH and N,N,N′,N′ -tetramethyl- p -phenylenediamine served as electron donors for Mo 6+ reduction. When NADH was used as an electron donor, at first cytochrome b in the cell extract was reduced, and then molybdenum blue was formed. Sodium cyanide strongly inhibited Mo 6+ reduction by NADH (5 mM) but not the reduction of cytochrome b in the cell extract, suggesting that the reduced component of the electron transport system after cytochrome b serves as an electron donor for Mo 6+ reduction. Both ferric and stannous ions strongly enhanced the activity of Mo 6+ reduction by NADH.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/aem.59.4.1176-1180.1993
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1993
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
