In:
Journal of Bacteriology, American Society for Microbiology, Vol. 153, No. 3 ( 1983-03), p. 1361-1367
Abstract:
By using localized mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, we isolated 39 temperature-sensitive growth mutants that exhibited high mutability when the bacteria were grown at the permissive temperature. Two of the mutations, dnaQ186 and dnaQ231, were shown to be new alleles of the dnaQ gene by genetic mapping and complementation tests with the dnaQ49 mutation previously isolated. They shared common properties with the dnaQ49 strain, but their mutator activity was not temperature dependent. The dnaQ mutants exhibited increased sensitivity to inhibitors of DNA gyrase and to DNA intercalating and alkylating agents.
Type of Medium:
Online Resource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/jb.153.3.1361-1367.1983
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1983
detail.hit.zdb_id:
1481988-0
SSG:
12