In:
Journal of Virology, American Society for Microbiology, Vol. 12, No. 4 ( 1973-10), p. 766-774
Abstract:
Biologically active herpes simplex virus (HSV)-induced antigens were selectively removed from extracts of infected BHK cells by affinity chromatography by utilizing an insoluble form of concanavalin A (Con A). Soluble extracts of 3 H-glucosamine-labeled, HSV-infected cells were absorbed to a Con A column. Bound material was eluted with α-methyl- d -mannoside (αMM) and NaCl. The specific activity of the eluted glycoproteins increased by 10-fold. Two broad groups of viral-induced antigens were isolated from Con A. Group I includes two antigens which bind to Con A by a specific mechanism because the antigens are dissociated by αMM. Group II contains three antigens which bind to Con A but apparently by a nonspecific or electrolytic mechanism. One antigen in group I was identified as the glycoprotein antigen, CP-1, described previously.
Type of Medium:
Online Resource
ISSN:
0022-538X
,
1098-5514
DOI:
10.1128/jvi.12.4.766-774.1973
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1973
detail.hit.zdb_id:
1495529-5
