In:
mBio, American Society for Microbiology, Vol. 6, No. 6 ( 2015-12-31)
Abstract:
Mycobacterium haemophilum is an emerging pathogen with an unknown natural reservoir that exhibits unique requirements for iron supplementation to grow in vitro . Understanding the basis for this iron requirement is important because it is fundamental to isolation of the organism from clinical samples and environmental sources. Defining the molecular basis for M. haemophilium 's growth requirements will also shed new light on mycobacterial strategies to acquire iron and can be exploited to define how differences in such strategies influence pathogenesis. Here, through a combination of sequencing and experimental approaches, we explain the basis for the iron requirement. We further demonstrate the genetic closeness of M. haemophilum and Mycobacterium leprae , the causative agent of leprosy which cannot be cultured in vitro , and we demonstrate methods to genetically manipulate M. haemophilum . These findings pave the way for the use of M. haemophilum as a model to elucidate functions of genes shared with M. leprae .
Type of Medium:
Online Resource
ISSN:
2161-2129
,
2150-7511
DOI:
10.1128/mBio.01313-15
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2015
detail.hit.zdb_id:
2557172-2