In:
mBio, American Society for Microbiology, Vol. 7, No. 1 ( 2016-03-02)
Kurzfassung:
Ebola virus (EBOV) causes outbreaks of highly lethal disease for which no approved vaccines or treatments exist. Recent work has elucidated key molecular features of the complex EBOV entry process, including stepwise interactions with multiple host factors. However, there is a critical gap in our understanding of events that surround the final membrane fusion step which persists due to the paucity of direct and extensive investigation of EBOV fusion. Here, we report a real-time assay for EBOV glycoprotein fusion triggering and use it to define its cellular location and requirements. We also uncover an unexpected requirement for host proteases at a step after fusion triggering that may reflect their role in formation of fusion pores for genome delivery.
Materialart:
Online-Ressource
ISSN:
2161-2129
,
2150-7511
DOI:
10.1128/mBio.01857-15
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
2016
ZDB Id:
2557172-2
