In:
Genome, Canadian Science Publishing, Vol. 53, No. 7 ( 2010-07), p. 568-574
Abstract:
We captured the whole human exome by hybridization using synthesized oligonucleotides, based on a high-density microarray design, and we sequenced those captured human exons using high-throughput sequencing on a Genome Sequencer FLX instrument. Of the uniquely mapped reads, 71% fell within target regions, and these corresponded to coverage of 94% of human genes, 87% of exons, and 70% of the total base-pair length of the CCDS set. Our study provides strong evidence for the practical usefulness of this method on a genome-wide scale, showing the resequenced whole human exome database with 501 microRNAs and 307 novel SNPs.
Type of Medium:
Online Resource
ISSN:
0831-2796
,
1480-3321
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2010
detail.hit.zdb_id:
2020635-5
SSG:
12