In:
Journal of Environmental Engineering and Science, Thomas Telford Ltd., Vol. 7, No. 5 ( 2008-09), p. 467-479
Abstract:
Polycyclic aromatic hydrocarbons (PAHs) are well-known carcinogens and mutagens. In this study, the biodegradation of PAHs (16 major pollutants mentioned by the US Environmental Protection Agency) present in industrial polluted soil was carried out under addition of different organic substrates such as pig manure, shrimp compost, or sewage sludge. Wastewater sludge and pig manure were used as inoculum to enhance biodegradation of PAHs. The results showed a maximum biodegradation of 60% of total PAHs in 16 weeks at 30 °C. This degradation rate was obtained for a biopile that consisted of 75% of PAH contaminated soil, 10% of inoculum (acclimatized sludge), 10% of crude sludge, and 3% of straw (to increase aeration rate). The ratio of soil–amendment was 1.0:0.3 and the C/N ratio was 30. In this assay, GC-MS results showed a degradation of 68% ± 16%.of PAH with five to six cycles. Quantification of PAH-dioxygenase genes in DNA extracted from soil and inoculum, using real-time polymerase chain reaction (RT-PCR) showed that the major bacterial degraders from the soil were Gram + bacteria, while in acclimated sludge (inoculum) major degraders were Gram – bacteria.
Type of Medium:
Online Resource
ISSN:
1496-2551
,
1496-256X
Language:
English
Publisher:
Thomas Telford Ltd.
Publication Date:
2008
detail.hit.zdb_id:
2074025-6