In:
Canadian Journal of Physiology and Pharmacology, Canadian Science Publishing, Vol. 91, No. 12 ( 2013-12), p. 1135-1142
Abstract:
Early growth response-1 one gene (Egr-1), one of the immediate early response genes, plays an important role in the adaptive response of the myocardium to hypertrophic stimuli. We aimed to investigate the effects of Egr-1 deletion on cardiac function. Egr-1 knock-out (Egr-1 −/− ) homozygous mice were employed to evaluate the electrophysiological and molecular properties of left ventricular cardiomyocytes (VCM) by using patch-clamp technique, intracellular calcium measurements, real-time PCR, and Western blot. Action potential was prolonged and diastolic potential was positive-shifted in VCMs isolated from Egr-1 −/− mice, in comparison with those from their wild-type (WT) littermates. The calcium content of the sarcoplasmic reticulum was reduced and the decay time for steady-state calcium transient slowed down. Serca2, Ryr, L-type Ca 2+ -channel, and PLB mRNA expression were reduced in Egr-1 −/− mice compared with the controls. Moreover, Serca2 protein was reduced, while the amount of Ncx1 protein was increased in Egr-1 −/− hearts compared with those of the WT littermates. Furthermore, genes involved in heart development (GATA-4, TGF-β) and in Egr-1 regulation (Nab1, Nab2) were down regulated in Egr-1 −/− mice. These results suggest that Egr-1 plays a pivotal role in regulating excitation–contraction coupling in cardiac myocytes.
Type of Medium:
Online Resource
ISSN:
0008-4212
,
1205-7541
DOI:
10.1139/cjpp-2012-0419
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2013
detail.hit.zdb_id:
2004356-9
