In:
Genome, Canadian Science Publishing, Vol. 59, No. 7 ( 2016-07), p. 501-507
Abstract:
ADP-glucose pyrophosphorylase (AGP), which consists of two large subunits (AGP-L) and two small subunits (AGP-S), controls the rate-limiting step in the starch biosynthetic pathway. In this study, a full-length open reading frame (ORF) of AGP-L gene (named as Agp2) in wheat and a series of Agp2 gene sequences in wheat relatives were isolated. The coding region of Agp2 contained 15 exons and 14 introns including a full-length ORF of 1566 nucleotides, and the deduced protein contained 522 amino acids (57.8 kDa). Generally, the phylogenetic tree of Agp2 indicated that sequences from A- and D-genome donor species were most similar to each other and sequences from B-genome donor species contained more variation. Starch accumulation and Agp2 expression in wheat grains reached their peak at 21 and 15 days post anthesis (DPA), respectively.
Type of Medium:
Online Resource
ISSN:
0831-2796
,
1480-3321
DOI:
10.1139/gen-2016-0007
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2016
detail.hit.zdb_id:
2020635-5
SSG:
12
