In:
Biochemistry and Cell Biology, Canadian Science Publishing, Vol. 64, No. 7 ( 1986-07-01), p. 699-705
Abstract:
A permanent human hybrid endothelial cell line (EA.hy926) was shown to produce the von Willebrand factor, a protein of 250 000 relative mass (M r ) which was secreted into the medium as a 220 000 M r protein. A cDNA library was constructed in λgt 11 using mRNA from these hybrid cells. Several von Willebrand factor cDNA clones were isolated from this library using a synthetic oligodeoxyribonucleotide as a hybridization probe. These cDNA clones were used to analyze the von Willebrand factor gene in normal individuals and in cultured cells.
Type of Medium:
Online Resource
ISSN:
0829-8211
,
1208-6002
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
1986
SSG:
12