In:
American Journal of Physiology-Renal Physiology, American Physiological Society, Vol. 298, No. 5 ( 2010-05), p. F1113-F1117
Kurzfassung:
Akt/PKB is known to regulate the facilitative glucose carrier GLUT4. Nothing is known, however, of the role of Akt/PKB in the regulation of renal epithelial transport. To explore whether Akt2/PKBβ influences the Na + -coupled glucose cotransporter SGLT1, human SGLT1 was expressed in Xenopus laevis oocytes with or without Akt/PKB, and electrogenic glucose transport was determined by dual-electrode voltage clamp. The coexpression of Akt/PKB in SGLT1-expressing oocytes was followed by an increase in glucose-induced currents. To study the functional significance of Akt/PKB-sensitive renal glucose transport, further experiments were performed in gene-targeted mice lacking functional Akt2/PKBβ ( akt2 −/− ) and in their wild-type littermates ( akt2 +/+ ). Plasma glucose concentration was significantly higher in akt2 −/− mice than in akt2 +/+ mice but was virtually identical to the plasma glucose concentration in fructose-treated akt2 +/+ mice. Urinary glucose excretion was significantly higher in akt2 −/− mice compared with akt2 +/+ mice with or without fructose treatment. Moreover, the glucose-induced depolarization of proximal tubular cells was significantly smaller in isolated, perfused renal tubules from akt2 −/− mice than in those from akt2 +/+ mice. In conclusion, Akt2/PKBβ plays a role in the regulation of renal glucose transport.
Materialart:
Online-Ressource
ISSN:
1931-857X
,
1522-1466
DOI:
10.1152/ajprenal.00592.2009
Sprache:
Englisch
Verlag:
American Physiological Society
Publikationsdatum:
2010
ZDB Id:
1477287-5
