In:
Journal of Biomedicine and Biotechnology, Hindawi Limited, Vol. 2012 ( 2012), p. 1-10
Abstract:
Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successful detection of a 35-base, single-strand nucleic acid target by Hall-based magnetic transduction as a mimic for pathogenic DNA target detection. The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350 nm superparamagnetic bead labeled with DNA. Detection of the target sequence was demonstrated at 364 pM ( 〈 2 target DNA strands per bead) target DNA in the presence of 36 μ M nontarget (noncomplementary) DNA ( 〈 10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic. The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point-of-care (POC) diagnostics and subsequent medical care.
Type of Medium:
Online Resource
ISSN:
1110-7243
,
1110-7251
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2012
detail.hit.zdb_id:
2698540-8
detail.hit.zdb_id:
2512507-2
SSG:
12