In:
PPAR Research, Hindawi Limited, Vol. 2014 ( 2014), p. 1-10
Abstract:
Objective . 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) reduces inflammation and has been identified as an anti-inflammatory prostaglandin in numerous animal models. In this study, we investigated both effects of 15d-PGJ2 and its protection mechanism in concanavalin A- (ConA-) induced autoimmune hepatitis in mice. Materials and Methods . In vivo, Balb/C mice were injected with ConA (25 mg/kg) to induce acute autoimmune hepatitis, and 15d-PGJ2 (10 μ g or 25 μ g) was administered 1 h before the ConA injection. The histological grade, proinflammatory cytokine levels, and NF- κ B and PPAR γ activity were determined 6, 12, and 24 h after the ConA injection. In vitro, LO2 cells and RAW264.7 cells were pretreated with 15d-PGJ2 (2 μ M) 1 h before the stimulation with ConA (30 μ g/mL). The NF- κ B and PPAR γ activity were determined 30 min after the ConA administration. Results . Pretreatment with 15d-PGJ2 reduced the pathological effects of ConA-induced autoimmune hepatitis and significantly reduced the levels of cytokines after injection. 15d-PGJ2 activated PPAR γ , blocked the degradation of I κ B α , and inhibited the translocation of NF- κ B into the nucleus. Conclusion . These results indicate that 15d-PGJ2 protects against ConA-induced autoimmune hepatitis by reducing proinflammatory cytokines. This reduction in inflammation may correlate with the activation of PPAR γ and the reduction in NF- κ B activity.
Type of Medium:
Online Resource
ISSN:
1687-4757
,
1687-4765
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2014
detail.hit.zdb_id:
2237981-2