In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2014 ( 2014), p. 1-7
Abstract:
Tectorigenin (TTR) is an O-methylated isoflavone derived from the rhizome of Belamacanda chinensis (L.) DC. It is known to perform a wide spectrum of biological activities such as antioxidant, anti-inflammatory, anti-tumor. The aim of this study is to examine the mechanism of antibacterial activity of TTR against methicillin-resistant Staphylococcus aureus (MRSA). The anti-MRSA activity of TTR was analyzed in combination assays with detergent, ATPase inhibitors, and peptidoglycan (PGN) derived from S. aureus . Transmission electron microscopy (TEM) was used to monitor survival characteristics and changes in S. aureus morphology. The MIC values of TTR against all the tested strains were 125 μ g/mL. The OD(600) of each suspension treated with a combination of Triton X-100, DCCD, and NaN 3 with TTR (1/10 × MIC) had been reduced from 68% to 80%, compared to the TTR alone. At a concentration of 125 μ g/mL, PGN blocked antibacterial activity of TTR. This study indicates that anti-MRSA action of TTR is closely related to cytoplasmic membrane permeability and ABC transporter, and PGN at 125 μ g/mL directly bind to and inhibit TTR at 62.5 μ g/mL. These results can be important indication in study on antimicrobial activity mechanism against multidrug resistant strains.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2014
detail.hit.zdb_id:
2148302-4