In:
Journal of Immunology Research, Hindawi Limited, Vol. 2015 ( 2015), p. 1-9
Abstract:
The activated NF- κ B signaling pathway plays an important role in pathogenesis of primary Sjögren’s syndrome (pSS). The inhibitor of κ B (I κ B) kinase (IKK) family such as IKK α , IKK β , IKK γ , and IKK ε , is required for this signaling. Our aim was to investigate the role of IKK α / β / γ / ε in patients with untreated pSS. In minor salivary glands from pSS patients, phosphorylated IKK ε (pIKK ε ), pI κ B α , and pNF- κ B p65 (p-p65) were highly expressed in ductal epithelium and infiltrating mononuclear cells by immunohistochemistry, compared to healthy individuals. pIKK α / β and pIKK γ were both negative. And pIKK ε positively related to expression of p-p65. Furthermore, pIKK ε and p-p65 expression significantly correlated with biopsy focus score and overall disease activity. Meanwhile, in peripheral blood mononuclear cells from pSS patients, pIKK ε , total IKK ε , pIKK α / β , and p-p65 were significantly increased by western blot, compared to healthy controls. However, there was no difference in IKK γ and I κ B α between pSS patients and healthy individuals. These results demonstrated an abnormality of IKK ε , I κ B α , and NF- κ B in pSS, suggesting a potential target of treatment for pSS based on the downregulation of IKK ε expression and deregulation of NF- κ B pathway.
Type of Medium:
Online Resource
ISSN:
2314-8861
,
2314-7156
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2015
detail.hit.zdb_id:
2817541-4
