In:
Stem Cells International, Hindawi Limited, Vol. 2019 ( 2019-12-19), p. 1-14
Kurzfassung:
The pulpotomy with pulp capping is aimed at retaining vital pulp with reparative dentin formation. Vascular endothelial growth factor (VEGF) plays a crucial role in dentin regeneration; however, its constant administrations in the human body is still problematic. Chitosan was widely studied as an effective carrier to deliver bioactive molecules in regenerative medicine. In this study, we conducted a chitosan/ β -glycerophosphate (CS/ β -GP) hydrogel as a VEGF-sustained release system and explored its effects on dental pulp stem cells (DPSCs). CS/ β -GP hydrogel was manufactured using a sol-gel method. SEM assay showed the spongy and porous microstructure of the lyophilized hydrogels. DPSCs cultured in the CS/ β -GP hydrogel kept adhesion and vitality. CCK-8 assay tested the promoted proliferation activity of DPSCs on the hydrogel. Besides, the added VEGF protein could continually release from VEGF/CS/ β -GP hydrogel. The VEGF/CS/ β -GP hydrogel could promote the odontogenic differentiation of DPSCs better than VEGF treatment without hydrogel. Our results suggested that CS/ β -GP hydrogel could continually release VEGF and contribute to odontogenic differentiation of DPSCs, thus may become a potential carrier of bioactive molecules in pulp capping therapy.
Materialart:
Online-Ressource
ISSN:
1687-966X
,
1687-9678
DOI:
10.1155/2019/1515040
Sprache:
Englisch
Verlag:
Hindawi Limited
Publikationsdatum:
2019
ZDB Id:
2573856-2
