In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 75, No. 15_Supplement ( 2015-08-01), p. 181-181
Abstract:
Head and neck squamous cell carcinoma (HNSCC) is one of the prevalent malignancies in the world. microRNAs (miRNAs) are involved in the neoplastic process of various malignancies including HNSCC to mediate post-transcriptional down-regulation of target genes. This study shows that miR-134 enhances the proliferation, migration and invasion of HNSCC cells. In silico modules predict that programmed cell death 7 (PDCD7) gene could be one of the targets of miR-134. In stable HNSCC cell subclones, ectopic miR-134 expression decreased PDCD7 expression; while the ectopic miR-134Zip expression increased PDCD7 expression. Reporter assays confirmed the binding of miR-134 to the wild type 3′UTR of PDCD7, which decreased reporter activity. The targeting of miR-134 to the 3′UTR of PDCD7 was abolished by the mutations in targeted sequences. Moreover, miR-134Zip expression increased the activity of wild type reporter. Down-regulation of PDCD7 was identified in around 70% of HNSCC tumors, which was significantly opposite to miR-134 expression. A trend of reverse expression between miR-134 and PDCD7 was also present in HNSCC cell lines. PDCD7 was up-regulated following the apoptosis induced by cis-platin. Ectopic PDCD7 expression decreased the proliferation and migration of HNSCC cells, but it was unable to affect apoptosis or drug resistance status. Moreover, miR-134 associated oncogenicity was attenuated by PDCD7 expression. Taking together, this study concludes that the oncogenic potential of miR-134 in head and neck carcinoma is able to be mediated by targeting PDCD7. Citation Format: Shih-Yuan Peng, Kuo-Wei Chang, Shu-Chun Lin, Hsi-Feng Tu. miR-134 targets programmed cell death 7 (PDCD7) gene to modulate the pathogenesis of head and neck carcinoma. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 181. doi:10.1158/1538-7445.AM2015-181
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2015-181
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2015
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
