In:
Oncology, S. Karger AG, Vol. 63, No. 4 ( 2002), p. 378-384
Abstract:
〈 i 〉 Objective: 〈 /i 〉 Human macrophage metalloelastase is referred to as matrix metalloproteinase (MMP-12), its function in tumors is contradictory. The current study was undertaken to investigate the role of MMP-12 in esophageal squamous cell carcinoma (SCC). 〈 i 〉 Patients and Methods: 〈 /i 〉 We analyzed the levels of MMP-12 mRNA expression in 67 patients with primary esophageal SCC by Northern blot analysis and the tissues were subjected to in situ hybridization analysis for MMP-12. Immunohistochemical staining was performed to detect the macrophages infiltrated in esophageal SCCs. 〈 i 〉 Results: 〈 /i 〉 MMP-12 mRNA was detected in 27 of 67 esophageal SCC samples by Northern blot analysis. In situ hybridization and immunohistochemical staining revealed that MMP-12 mRNA signals are located mainly in tumor cells. The frequency of lymph node metastasis was significantly higher in the MMP-12-positive (MMP-12(+)) subgroup than MMP-12-negative (MMP-12(–)) subgroup (p 〈 0.05); furthermore, invasion was significantly deeper in the MMP- 12(+) subgroup than in the MMP-12(–) subgroup (p 〈 0.01). MMP-12 mRNA was inversely correlated with prognosis (p 〈 0.05). However, Cox multivariate analysis revealed that upregulation of MMP-12 was not related to prognosis. 〈 i 〉 Conclusions: 〈 /i 〉 MMP-12 gene expression was associated with the progression of esophageal SCC; however, it was not an independent prognostic factor.
Type of Medium:
Online Resource
ISSN:
0030-2414
,
1423-0232
Language:
English
Publisher:
S. Karger AG
Publication Date:
2002
detail.hit.zdb_id:
1483096-6
detail.hit.zdb_id:
250101-6
