In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 140, No. 4 ( 2006), p. 315-320
Kurzfassung:
〈 i 〉 Background: 〈 /i 〉 Desloratadine is an H 〈 sub 〉 1 〈 /sub 〉 -histamine antagonist which possesses additional anti-inflammatory properties through inhibition of leukocyte activation and reduction of ICAM-1 expression on mucosal epithelial cells. So far no studies have addressed the potential anti-inflammatory activities of desloratadine and loratadine on skin keratinocytes. 〈 i 〉 Objective: 〈 /i 〉 In this study the capacity of desloratadine and loratadine to counteract human keratinocyte activation by interferon-γ (IFN-γ) was analyzed. In particular, the chemokine release of kerationcytes and the crosstalk between keratinocytes and lymphocytes were examined. 〈 i 〉 Method: 〈 /i 〉 Keratinocyte cultures established from normal skin of healthy donors were activated by IFN-γ in the absence or presence of desloratadine and loratadine, and tested for the release of CCL5/RANTES, CXCL8/IL-8, CCL17/TARC and CXCL10/IP-10. Furthermore the supernatants of differentially stimulated keratinocytes were used for migration studies of human neutrophils, eosinophils and polarized Th1/Th2 clones. 〈 i 〉 Results: 〈 /i 〉 Desloratadine and loratadine inhibited the constitutive and IFN-γ-induced release of CCL5, CXCL8 and CXCL10 from keratinocytes, while the low release of CCL17 remained unchanged. Furthermore the crosstalk between lymphocytes and keratinocytes was blocked as shown by a reduced capacity of desloratadine/loratadine-stimulated keratinocytes to attract human neutrophils, eosinophils and T cells. 〈 i 〉 Conclusions: 〈 /i 〉 The results indicate that desloratadine has the capacity to block the IFN-γ-induced activation of keratinocytes, and that it can thus exert important regulatory effects on cell-mediated immune responses in the skin. The rather high doses required for these effects argue for a topical application when trying to use desloratadine in epidermal inflammatory conditions.
Materialart:
Online-Ressource
ISSN:
1018-2438
,
1423-0097
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2006
ZDB Id:
1482722-0
