In:
Intervirology, S. Karger AG, Vol. 50, No. 4 ( 2007), p. 303-309
Kurzfassung:
〈 i 〉 Objective: 〈 /i 〉 To screen and identify cellular proteins binding to the core region of hepatitis C virus (HCV) RNA genome. 〈 i 〉 Methods: 〈 /i 〉 The plasmid pHCV core was constructed to generate in vitro transcripts of the core region of HCV RNA genome. Ultraviolet (UV) cross-linking experiment and competition analysis were performed to screen HepG2 cellular proteins, which interact with digoxin-labeled transcripts of the core region of HCV RNA genome. RNA-binding proteins were separated by immunoprecipitation, analyzed by electrophoresis on SDS-PAGE and detected by immunoblotting with anti-digoxingenin-AP. After being excised from SDS-PAGE, the proteins bands were analyzed by MALDI-TOF-MS. 〈 i 〉 Results: 〈 /i 〉 Several cellular proteins of hepG2 cell specifically bound to the core region of HCV RNA genome. The binding of cellular proteins to digoxin-labeled HCV core RNA was competed out in proportion to the increasing amount of unlabeled RNA. One of the HCV RNA-binding proteins was the B (brain) isozyme of human phosphoglycerate mutase (PGAM-B) identified by MALDI-TOF-MS. 〈 i 〉 Conclusion: 〈 /i 〉 PGAM-B could specifically bind to the core region of HCV RNA genome in vitro.
Materialart:
Online-Ressource
ISSN:
0300-5526
,
1423-0100
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2007
ZDB Id:
1482863-7
SSG:
12
