In:
Cells Tissues Organs, S. Karger AG, Vol. 186, No. 4 ( 2007), p. 213-220
Kurzfassung:
The transcription factor 〈 i 〉 Ets2 〈 /i 〉 and its transcriptional targets osteopontin (OPN) and osteocalcin (OC) are expressed in tissue-engineered bone constructs in vitro. Up to now little is known about the role of 〈 i 〉 Ets2 〈 /i 〉 in tissue-engineering applications. This study was intended to investigate the hypothesis that protein expression of 〈 i 〉 Ets2 〈 /i 〉 is correlated with the expression of bone-specific proteinsin tissue-engineeredbone constructs. Cell-seeded three-dimensional bone constructs manufactured with osteoblastic cells and poly(lactic-co-glycolic acid) polymer fleeces over a period of 21 days were analyzed by SDS-PAGE and Western blotting. The protein expression of OPN, OC, osteonectin and collagen type I was analyzed. Cellularity, alkaline phosphatase-specific activity and histology confirmed the osteoblastic phenotype of the constructs. Correlations between 〈 i 〉 Ets2 〈 /i 〉 expression and OPN and 〈 i 〉 Ets2 〈 /i 〉 and collagen type I expression could be detected during the phase of late osteoblastic differentiation between days 9 and 21. The correlation between OC and collagen type I was significant in this late stage of osteoblastic differentiation. These results suggest that there is a strong interplay of 〈 i 〉 Ets2 〈 /i 〉 with bone-specific proteins in cell-seeded three-dimensional bone constructs. This study is a crucial step to elucidate the complex interplay of bone-related proteins in the application of bone tissue engineering.
Materialart:
Online-Ressource
ISSN:
1422-6405
,
1422-6421
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2007
ZDB Id:
1481840-1
SSG:
12
