In:
European Surgical Research, S. Karger AG, Vol. 40, No. 1 ( 2008), p. 1-6
Kurzfassung:
〈 i 〉 Background/Aims: 〈 /i 〉 Volatile anesthetics are frequently utilized in clinical routine. Isoflurane has been shown to attenuate the response to inflammatory stimuli such as lipopolysaccharide (LPS) when administered 〈 i 〉 before 〈 /i 〉 induction of endotoxemia. We aimed therefore to evaluate the effect of isoflurane 〈 i 〉 after 〈 /i 〉 administration of LPS on the cytokine release as a therapeutic option. 〈 i 〉 Materials and Methods: 〈 /i 〉 21 male Sprague-Dawley rats were randomly assigned to the following groups: animals that received LPS (5 mg/kg, i.v.) without further intervention (LPS group), animals that received continuous inhalation of 1 minimum alveolar concentration (MAC) isoflurane 15 min after administration of LPS (Iso group) and no specific intervention (sham group). Four hours following LPS injection, plasma levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-10 were determined. Furthermore, nitrite release from cultured alveolar macrophages was analyzed. 〈 i 〉 Results: 〈 /i 〉 Inhalation of isoflurane after induction of endotoxemia attenuated the release of TNF-α (–52%, p 〈 0.05) and IL-1β (–39%, p 〈 0.05) as compared to the LPS group, while IL-6 and IL-10 levels were not significantly altered. Nitrite release was significantly increased in the Iso group as compared to the LPS group (+115%, p 〈 0.05). 〈 i 〉 Conclusion: 〈 /i 〉 Inhalation of 1 MAC isoflurane after induction of endotoxemia in rats attenuates the systemic release of proinflammatory cytokines and concurrently enhances the production of nitrite in cultured alveolar macrophages.
Materialart:
Online-Ressource
ISSN:
0014-312X
,
1421-9921
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2008
ZDB Id:
1468505-X
