In:
Pharmacology, S. Karger AG, Vol. 82, No. 3 ( 2008), p. 228-232
Abstract:
Inhibitory synaptic transmission within the dorsal horn of the spinal cord plays a key role in the processing of nociceptive signals, and mainly involves glycine. We have studied the effects of alphaxalone on α 〈 sub 〉 1 〈 /sub 〉 homomeric glycine receptors expressed in a mammalian expression system (HEK 293 cells) using the whole-cell patch-clamp technique. Our experiments showed a coactivating effect of alphaxalone with a concentration for half-maximum activation (EC 〈 sub 〉 50 〈 /sub 〉 ) of the effect of a low glycine concentration (EC 〈 sub 〉 20 〈 /sub 〉 ) of 70.9 ± 21.5 µmol/l. Taking into account the results of other groups, our study suggests that neuroactive steroids might be an interesting class of compounds to probe subunit-specific effects of glycine receptors.
Type of Medium:
Online Resource
ISSN:
0031-7012
,
1423-0313
Language:
English
Publisher:
S. Karger AG
Publication Date:
2008
detail.hit.zdb_id:
1483550-2
SSG:
15,3