In:
Chemotherapy, S. Karger AG, Vol. 58, No. 3 ( 2012), p. 195-199
Kurzfassung:
〈 b 〉 〈 i 〉 Aims: 〈 /i 〉 〈 /b 〉 This study aimed to evaluate the different abilities of various 〈 i 〉 Phellinus igniarius 〈 /i 〉 strains inhibiting quorum sensing (QS), to search for novel QS inhibitors from them and to analyze their inhibitory activity, with a view to their possible use in controlling infections. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 The bioactive metabolites produced by 〈 i 〉 P. igniarius 〈 /i 〉 cultures were tested for their abilities to inhibit QS-regulated behavior. All 〈 i 〉 P. igniarius 〈 /i 〉 strains were cultured in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2-fold volumes by freeze drying. The condensed supernatant was sterilized by filtration through a 0.22-µm membrane filter and added to 〈 i 〉 Chromobacterium violaceum 〈 /i 〉 CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The bioactive metabolites produced by 10 〈 i 〉 P. igniarius 〈 /i 〉 strains could inhibit violacein production, a QS-regulated behavior in 〈 i 〉 C. violaceum 〈 /i 〉 . Furthermore, these strains could be roughly categorized into three groups on the basis of their inhibitory activities. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 〈 i 〉 P. igniarius 〈 /i 〉 strains can produce QS-inhibitory compounds and have different abilities to inhibit QS.
Materialart:
Online-Ressource
ISSN:
0009-3157
,
1421-9794
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2012
ZDB Id:
1482111-4
SSG:
15,3
