In:
Respiration, S. Karger AG, Vol. 88, No. 3 ( 2014), p. 251-261
Abstract:
〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Diagnosis and treatment monitoring of patients with tuberculosis remain challenging. 〈 b 〉 〈 i 〉 Objective: 〈 /i 〉 〈 /b 〉 We have evaluated whether 〈 i 〉 Mycobacterium 〈 /i 〉 -specific interferon (IFN)- & #947; and interleukin (IL)-2 bifunctional cytokine immune response assays improve the diagnosis of and correlate to treatment response in pulmonary tuberculosis. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Early secretory antigenic target (ESAT)6/culture filtrate protein 10 (CFP10), microsomal triglyceride transfer protein 65 (MTP65) and the purified protein derivative (PPD) tuberculin-specific immune profiles were investigated in peripheral blood mononuclear cells from 19 patients with culture-confirmed tuberculosis and 23 healthy community controls (HCCs; 82.6% with latent 〈 i 〉 M. tuberculosis 〈 /i 〉 infection) using a novel fluorescence-based dual-colour enzyme-linked immunospot (EliSpot) technology (FluoroSpot). 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The frequency of ESAT6/CFP10-induced IFN- & #947;+IL-2- producing cells was elevated (p 〈 0.001), whereas the percentages of specific IFN- & #947;-IL-2+ (p = 0.002) and IFN- & #947;+IL-2+ double producing cells (p = 0.037) were diminished in tuberculosis patients in comparison to HCCs. A 3-host marker model using a combination of those IFN- & #947; and IL-2 single-cell responses showed 93.8% sensitivity and 77.8% specificity for tuberculosis. During tuberculosis treatment, the PPD-induced immune responses shifted from an IFN- & #947;+IL-2- dominated profile towards a balance of IFN- & #947;-IL-2+ and IFN- & #947;+IL-2+ double producing cells (all p ≤ 0.05). 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 The addition of antigen-specific IL-2 production to IFN- & #947; responses by EliSpot in IFN- & #947; release assays increases diagnostic sensitivity for active tuberculosis.
Type of Medium:
Online Resource
ISSN:
0025-7931
,
1423-0356
Language:
English
Publisher:
S. Karger AG
Publication Date:
2014
detail.hit.zdb_id:
1464419-8
