In:
Journal of Innate Immunity, S. Karger AG, Vol. 8, No. 5 ( 2016), p. 452-463
Abstract:
Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract infections. Immunity to RSV is initiated upon detection of the virus by pattern recognition receptors, such as RIG-I-like receptors. RIG-I-like receptors signal via MAVS to induce the synthesis of proinflammatory mediators, including type I interferons (IFNs), which trigger and shape antiviral responses and protect cells from infection. Alveolar macrophages (AMs) are amongst the first cells to encounter invading viruses and the ones producing type I IFNs. However, it is unclear whether IFNs act to prevent AMs from serving as vehicles for viral replication. In this study, primary AMs from MAVS ( 〈 i 〉 Mavs 〈 /i 〉 〈 sup 〉 -/- 〈 /sup 〉 )- or type I IFN receptor ( 〈 i 〉 Ifnar1 〈 /i 〉 〈 sup 〉 -/- 〈 /sup 〉 )-deficient mice were exposed to RSV ex vivo. Wild-type (wt) AMs but not 〈 i 〉 Mavs 〈 /i 〉 〈 sup 〉 -/- 〈 /sup 〉 and 〈 i 〉 Ifnar1 〈 /i 〉 〈 sup 〉 -/- 〈 /sup 〉 AMs produced inflammatory mediators in response to RSV. Furthermore, 〈 i 〉 Mavs 〈 /i 〉 〈 sup 〉 -/- 〈 /sup 〉 and 〈 i 〉 Ifnar1 〈 /i 〉 〈 sup 〉 -/- 〈 /sup 〉 AMs accumulated more RSV proteins than wt AMs, but the infection was abortive. Thus, RIG-I-like receptor-MAVS and IFNAR signalling are important for the induction of proinflammatory mediators from AMs upon RSV infection, but this signalling is not central for controlling viral replication. The ability to restrict viral replication makes AMs ideal sensors of RSV infection and important initiators of immune responses in the lung.
Type of Medium:
Online Resource
ISSN:
1662-811X
,
1662-8128
Language:
English
Publisher:
S. Karger AG
Publication Date:
2016
detail.hit.zdb_id:
2455818-7
