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    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2003
    In:  Circulation Vol. 108, No. 10 ( 2003-09-09), p. 1253-1258
    In: Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 108, No. 10 ( 2003-09-09), p. 1253-1258
    Abstract: Background— Oxidative stress seems to be present in all forms of hypertension. Thus, we tested the hypothesis that high intraluminal pressure (P i ) itself, by activating vascular oxidases, elicits increased superoxide (O 2 ·− ) production interfering with flow-induced dilation. Methods and Results— Isolated, cannulated rat femoral arterial branches were exposed in vitro (for 30 minutes) to normal P i (80 mm Hg) or high P i (160 mm Hg). High P i significantly increased vascular O 2 ·− production (as measured by lucigenin chemiluminescence and ethidium bromide fluorescence) and impaired endothelium-dependent dilations to flow; these effects could be reversed by superoxide dismutase. Administration of the NAD(P)H oxidase inhibitor diphenyleneiodonium, apocynin, the protein kinase C (PKC) inhibitor chelerythrine or staurosporin or the removal of extracellular Ca 2+ during high P i treatment prevented the increases in O 2 ·− production, whereas administration of losartan or captopril had no effect. High P i resulted in significant increases in intracellular Ca 2+ ([Ca 2+ ] i ) in the vascular wall (fura 2 fluorescence) and phosphorylation of PKCα (Western blotting). The PKC activator phorbol myristate acetate significantly increased vascular O 2 ·− production, which was inhibited by superoxide dismutase, diphenyleneiodonium, chelerythrine, or removal of extracellular Ca 2+ . Both high P i and phorbol myristate acetate increased the phosphorylation of the NAD(P)H oxidase subunit p47 phox . Conclusion— High P i itself elicits arterial O 2 ·− production, most likely by PKC-dependent activation of NAD(P)H oxidase, thus providing a potential explanation for the presence of oxidative stress and endothelial dysfunction in various forms of hypertension and the vasculoprotective effect of antihypertensive agents of different mechanisms of action.
    Type of Medium: Online Resource
    ISSN: 0009-7322 , 1524-4539
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2003
    detail.hit.zdb_id: 1466401-X
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