In:
Arteriosclerosis, Thrombosis, and Vascular Biology, Ovid Technologies (Wolters Kluwer Health), Vol. 33, No. 8 ( 2013-08), p. 1820-1828
Abstract:
The purpose of this study was to investigate the influence of cGMP-dependent kinase I (cGKI) on platelet production. Approach and Results— We used hematology analyser to measure platelet counts in conventional cGKI-null mutants (cGKI L1/L1 ), gene-targeted cGKIα/β rescue mice (referred to as cGKI-smooth muscle [SM]) in which cGKI expression is specifically restored only in SM, platelet factor 4-Cre tg/+ ; cGKI L2/L2 mice in which the cGKI protein was specifically deleted in the megakaryocyte/platelet lineage and cGKI-deficient bone marrow–chimeras. Thrombocytosis was detected in cGKI L1/L1 and in cGKI-SM. In contrast, neither platelet factor 4-Cre tg/+ ; cGKI L2/L2 nor cGKI-deficient bone marrow–chimeras displayed a thrombocytosis phenotype, indicating that the high platelet count in cGKI L1/L1 and cGKI-SM mutants is attributable to loss of an extrinsic signal rather than reflecting an intrinsic defect in megakaryopoiesis. Cytometric analyses further showed that stimulation of bone marrow–derived wild-type megakaryocytes in vitro using serum preparations obtained from cGKI-SM mutants strongly accelerated megakaryopoiesis, suggesting that the high platelet count develops in response to serum factors. Indeed, using ELISA assay, we found elevated levels of interleukin-6, a known stimulator of thrombopoiesis, in cGKI-SM mutant serum, whereas interleukin-6 levels were unaltered in platelet factor 4-Cre tg/+ ; cGKI L2/L2 mice and cGKI-deficient bone marrow–chimeras. Accordingly, antibody-mediated blockade of interleukin-6 normalized platelet counts in cGKI-SM mice. Conclusions— Abnormal cGMP/cGKI signaling in nonhematopoietic cells affects thrombopoiesis via elevated interleukin-6 production and results in thrombocytosis in vivo. Dysfunction of cGMP/cGKI signaling in nonhematopoietic cells contributes to a high platelet count, which is potentially associated with thrombosis.
Type of Medium:
Online Resource
ISSN:
1079-5642
,
1524-4636
DOI:
10.1161/ATVBAHA.113.301507
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2013
detail.hit.zdb_id:
1494427-3
