In:
Stroke, Ovid Technologies (Wolters Kluwer Health), Vol. 46, No. 4 ( 2015-04), p. 1127-1131
Abstract:
In spite of its high disease burden, there is no specific treatment for multi-infarct dementia. The preclinical evaluation of candidate drugs is limited because an appropriate animal model is lacking. Therefore, we aimed to evaluate whether a mouse model of recurrent photothrombotic stroke is suitable for the preclinical investigation of multi-infarct dementia. Methods— Recurrent photothrombotic cortical infarcts were induced in 25 adult C57BL/6 mice. Twenty-five sham-operated animals served as controls. The object recognition test and the Morris water maze test were performed 〉 6 weeks to assess cognitive deficits. Afterward, histological analyses were performed to characterize histopathologic changes associated with recurrent photothrombotic infarcts. Results— After the first infarct, the object recognition test showed a trend toward an impaired formation of recognition memories ( P =0.08), and the Morris Water Maze test revealed significantly impaired spatial learning and memory functions ( P 〈 0.05). After recurrent infarcts, the object recognition test showed significant recognition memory deficits ( P 〈 0.001) and the Morris water maze test demonstrated persisting spatial learning and memory deficits ( P 〈 0.05). Histological analyses revealed remote astrogliosis in the hippocampus. Conclusions— Our results show progressive cognitive deficits in a mouse model of recurrent photothrombotic stroke. The presented model resembles the clinical features of human multi-infarct dementia and enables the investigation of its pathophysiological mechanisms and the evaluation of treatment strategies.
Type of Medium:
Online Resource
ISSN:
0039-2499
,
1524-4628
DOI:
10.1161/STROKEAHA.115.008905
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2015
detail.hit.zdb_id:
1467823-8
